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Paper ID:IJSREM64828

AI-Driven Malware Obfuscation Detection and Defence System Using Ensemble Machine Learning and Byte-Level DNA Fingerprinting

Stream/Area:

-

Subject:

-

Paper ID:

IJSREM64828

Volume:

10 |

6 |

Jun 2026

Pages:

1-9

Publication Frequency:

Monthly (12 issues per year)

DOI
Digital Object Identifier
10.55041/IJSREM64828

License:International Journal of Scientific Research in Engineering and Management follows the Creative Commons Attribution 4.0 (CC BY 4.0) License, allowing free use and distribution with proper attribution.

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Authors

Manjusri SA

Manjusri SA

MSC CYBER FORENSIC INFORMATION SECURITY

DR. M.G.R EDUCATIONAL AND RESEARCH INSTITUTE UNIVERSITY

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Mentors

SNST

SHANKARA NARYANAN S T

MSC CYBER FORENSIC INFORMATION SECURITY

DR. M.G.R EDUCATIONAL AND RESEARCH INSTITUTE UNIVERSITY

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Abstract

Due to the increased utilization of malware obfuscation tactics, there is an ever-growing ineffectiveness of classic antivirus solutions that use the signature-based approach. Malware writers have been using different approaches such as packing, encoding, code tampering, and process obfuscation for avoiding detection by antivirus programs. To overcome this obstacle, MalDex AI, a new online malware analyser, will be proposed in this paper. It consists of the hybrid ensemble architecture that includes two algorithms: a Random Forest (RF) classifier and a Long Short-Term Memory (LSTM) algorithm. It performs the analysis of twenty behavioural and structural features found in the uploaded file and produces a final prediction based on a weighted sum of outputs of two algorithms, where RF constitutes 60%, and LSTM makes up 40%. In order to identify malware variants capable of evading signature-based detection, the system also comprises a byte DNA Fingerprinting module, which creates a byte bigram transition matrix (256 x 256) and heatmaps for discovering similarities within malware samples. Furthermore, the system utilizes Shannon entropy calculation in order to quantify file obfuscation, identify the use of UPX packaging, process hollowing, and code obfuscation techniques. Explainable Artificial Intelligence (XAI) technology is another functionality provided by MalDex AI. It allows for providing explanations behind detected threats as well as mapping suspicious behaviour to MITRE ATTACK techniques. Developed as a lightweight Flask application, MalDex AI can help to analyse malware efficiently and accurately. The efficiency of the tool was experimentally proven using a set of 4,000 malware samples.

KEYWORDS

Malware Detection Ensemble Learning Random Forest LSTM Byte DNA Fingerprinting Shannon Entropy Analysis Explainable AI (XAI) MITRE ATTACK Malware Obfuscation Cybersecurity.

Cite This Article

Manjusri SA, SHANKARA NARYANAN S T, "AI-Driven Malware Obfuscation Detection and Defence System Using Ensemble Machine Learning and Byte-Level DNA Fingerprinting," International Journal of Scientific Research in Engineering and Management, vol. 10, no. 6, pp. 1-9, Jun 2026. https://doi.org/10.55041/IJSREM64828

Full Article/Conclusion

  In this paper, MalDex AI is introduced — a web application to detect malware that is based exclusively on tackling the issue of obfuscation. It uses a combination of an RF + LSTM ensemble which can detect 100% of obfuscations on artificial data, alongside five specific tools dedicated to the identification of malware obfuscation: an entropy score, packer signatures, obfuscation types classification by name, suspicious strings scanning, and byte bigram DNA. The inclusion of a detector of attacks against the system and of an automatic MITRE ATTACK kill chain reconstructing tool sets it apart from all freely available systems. Testing was performed on FACT_Broucher_2026.pdf, and it showed a live detection with model scores matching those of a medium risk behavioural profile. Three directions will be pursued in future research. First, the models should be trained with an actual malware binary database from public resources for achieving realistic performance results. Secondly, instead of the current fake behavioural data generation approach, we can implement a true sandbox environment for malware execution using Docker containers. Finally, it is proposed to use the DNA-fingerprinting algorithm for cross file similarity searches that would allow to determine whether a newly uploaded file is related to any previous analysed samples within the same cyberattack campaign.

References

[1] AV-TEST Institute 2024, 'IT Security Report — Malware Statistics 2024', AV-TEST GmbH, viewed June 2026, <https://www.av-test.org>. [2] Gibert, D, Mateu, C and Planes, J 2020, 'The rise of machine learning for detection and classification of malware: Research developments, trends and challenges', Journal of Network and Computer Applications, vol. 153, p. 102526. [3] Aslan, O and Yilmaz, A.A 2021, 'A new malware classification framework based on deep learning algorithms', IEEE Access, vol. 9, pp. 87936-87951. [4] Ma, Y.W, Chen, J.L, Kuo, W.H and Chen, Y.C 2022, 'AI@nti-Malware: An intelligent framework for defending against malware attacks', Journal of Information Security and Applications, vol. 65, p. 103092. [5] Rokach, L 2019, 'Dynamic malware analysis in the modern era — A state of the art survey', ACM Computing Surveys, vol. 52, no. 5, pp. 1-48. [6] Lakshmi, T.S, Govindarajan, M and Sreenivasulu, A 2022, 'Malware visual resemblance analysis with minimum losses using Siamese neural networks', Theoretical Computer Science. [7] VirusTotal 2024, 'About VirusTotal', Google LLC, viewed June 2026, <https://www.virustotal.com/gui/about>. [8] Jamalpur, S, Navya, Y.S, Raja, P, Tagore, G and Reddy, G.R.K 2018, 'Dynamic malware analysis using Cuckoo Sandbox', Proc. 2018 ICSCCC, IEEE, pp. 1-4. [9] CrowdStrike 2024, 'Falcon Platform Overview', CrowdStrike Inc., viewed June 2026, <https://www.crowdstrike.com/products/>. [10] Maniriho, P, Mahmood, A.N and Chowdhury, M.J.M 2024, 'A Survey of Recent Advances in Deep Learning Models for Detecting Malware in Desktop and Mobile Platforms', ACM Computing Surveys, vol. 56, no. 1, pp. 1-41. [11] MITRE Corporation 2024, 'ATTACK for Enterprise — Techniques', MITRE ATTACK Knowledge Base, viewed June 2026, <https://attack.mitre.org>. [12] Cybersecurity Ventures 2024, 'Cybercrime To Cost The World $10.5 Trillion Annually By 2025', Cybersecurity Ventures Annual Report.  

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Publication Date

6/11/2026

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