ANALYTICAL METHOD DEVELOPMENT FOR PIPER BETEL LINN.

HPTLC method which is simple, particular and robust has been developed for the hydroxychavicol. The method was validated using parameters such as linearity, specificity, and precision, limit of quantification (LOQ), limit of detection (LOD), accuracy and robustness as per ICH guidelines. The present work deals with development of HPTLC method. Chromatographic separation of the drug was performed on Merck TLC aluminum plates pre-coated with silica gel 60F254 as the stationary phase. Butanol: Ethyl Acetate in the composition of 70:30 (v/v) were optimized for method development. The sample solutions were prepared in ethanol and linear ascending development was carried out in twin trough glass chamber and scanned at 280 nm using Camag TLC scanner. The marker was resolved successfully with Rf values 0.64±0.02 for hydroxychavicol respectively. The regression analysis data indicated good linear relationship for the calibration plots for hydroxychavicol in the range of 200-800 ng/spot and regression coefficient was 0.9997.