ANTI-FUNGAL ACTIVITY OF CALLUS BODY OF VERBESINA ENCELIOIDES LEAVES

Verbesina encelioides belongs to the Asteraceae family and also known as golden crown beard and wild sunflower. In present study callus was raised from the leaf explant of V. encelioides. Callus was obtained on MS medium. The callus was used for antifungal activity identification. In the in-vitro study of the anti-fungal activity Candida albicans gave the most effective response out of all the studied fungi, with best activity being displayed in methanol extract followed by hexane and ethyl acetate. With the best activity in ethyl acetate and hexane, and the least activity in methanol extract, Penicillium chrysogenum was the least responsive fungal species. In comparison to ethyl acetate and hexane extracts, Aspergillus niger displayed the greatest activity in methanol extract. In the presence of an ethyl acetate extract, Trichoderma reesei was effectively active, however, its activity decreased when exposed to methanol and hexane.