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ArticlePeer ReviewedOpen AccessPlagiarism Passed
Paper ID:IJSREM65662

Antifungal Activity of Fermented Extracts of Azadirachta indica, Allium sativum, Ocimum tenuiflorum, and Curcuma longa Against Fusarium oxysporum and Alternaria alternata

Stream/Area:

-

Subject:

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Paper ID:

IJSREM65662

Volume:

10 |

6 |

Jun 2026

Pages:

1-12

Publication Frequency:

Monthly (12 issues per year)

DOI
Digital Object Identifier
10.55041/IJSREM65662

License:International Journal of Scientific Research in Engineering and Management follows the Creative Commons Attribution 4.0 (CC BY 4.0) License, allowing free use and distribution with proper attribution.

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Authors

Shrikant Prakashrao Shinde

Shrikant Prakashrao Shinde

Department of Microbiology

Shri Datta Arts, Commerce and Science College, Hadgaon, Maharashtra, India.

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Shubham Chandramani Sawatkar

Shubham Chandramani Sawatkar

Department of Microbiology

Shankarrao Chavan Mahavidyalaya, Ardhapur, Nanded , Maharashtra, India.

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Abstract

Plant pathogenic fungi, particularly Fusarium oxysporum and Alternaria alternata, are responsible for significant crop losses worldwide, necessitating the development of environmentally sustainable alternatives to synthetic fungicides. The present study evaluated the antifungal activity of fermented ethanolic extracts of Azadirachta indica (Neem), Allium sativum (Garlic), Curcuma longa (Turmeric), and Ocimum tenuiflorum (Tulsi) against these two economically important phytopathogens. Plant materials were fermented in 10% molasses using Lactobacillus plantarum for 12, 24, 36, and 48 days, followed by ethanolic extraction. Antifungal efficacy was assessed using agar well diffusion, minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC), and GC–MS analysis. Fermentation significantly enhanced the antifungal activity of all plant extracts, with 48-day fermented extracts exhibiting the greatest inhibitory effects. Garlic extract demonstrated the highest antifungal activity, producing the largest inhibition zones of 28.6 ± 0.4 mm against F. oxysporum and 31.3 ± 0.4 mm against A. alternata at 50 mg mL⁻¹. The lowest MIC values were recorded for garlic and tulsi extracts, reaching 1.25 mg mL⁻¹ against F. oxysporum and 0.625 mg mL⁻¹ against A. alternata after 48 days of fermentation. GC–MS analysis identified several bioactive constituents, including diallyl disulfide, diallyl trisulfide, eugenol, ar-turmerone, β-turmerone, phytol, and β-caryophyllene, which are known for their antimicrobial properties. Statistical analysis revealed significant effects (P < 0.05) of plant species, fermentation period, extract concentration, and fungal pathogen on antifungal activity. These findings indicate that microbial fermentation enhances the bioactivity of medicinal plant extracts and highlight fermented garlic and tulsi extracts as promising eco-friendly biofungicidal candidates for the sustainable management of fungal plant diseases.

KEYWORDS

Allium sativum; Alternaria alternata; antifungal activity; biofungicide; fermentation; Fusarium oxysporum; GC–MS; medicinal plants; minimum inhibitory concentration (MIC); Ocimum tenuiflorum.

Cite This Article

Shrikant Prakashrao Shinde, Shubham Chandramani Sawatkar, "Antifungal Activity of Fermented Extracts of Azadirachta indica, Allium sativum, Ocimum tenuiflorum, and Curcuma longa Against Fusarium oxysporum and Alternaria alternata," International Journal of Scientific Research in Engineering and Management, vol. 10, no. 6, pp. 1-12, Jun 2026. https://doi.org/10.55041/IJSREM65662

Full Article/Conclusion

Overall, the study highlights the potential of fermented medicinal plant extracts as environmentally friendly alternatives to synthetic fungicides for the management of important plant pathogenic fungi. The enhanced antifungal activity observed following fermentation suggests that microbial biotransformation represents an effective strategy for improving the efficacy of botanical fungicides. Nevertheless, further investigations involving purification of individual bioactive compounds, quantitative metabolomic profiling, greenhouse validation, field evaluation, and toxicity assessment are required before large-scale agricultural application.

References

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Publication Date

6/30/2026

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