Biogenic Synthesis of Nanoparticles Using Secondary Metabolites from Pseudomonas fluorescence and its Antimicrobial Activity

Biogenic Synthesis of Nanoparticles Using Secondary Metabolites from Pseudomonas fluorescence and its Antimicrobial Activity.

Shubham Pate**¹², Shivani Yendhe*¹², Uthkarsha Kolekar², Omkar Dere², Moin Kazi².

¹Department of Microbiology, Samarth Institute of Paramedical Science, Pune, India

²Department of Microbiology, Samarth Institute of Pharmacy, Pune, India.

Abstract: Biogenic synthesis of Silver nanoparticles (AgNPs) using extracellular metabolites from the bacterium Pseudomonas fluorescence offers an eco-friendly and sustainable way of metal nanoparticle synthesis. The present work highlights the extracellular secondary metabolite, siderophore, produced by P. fluorescence mediated biotransformation of silver nitrate solution (1mM) into AgNP. AgNPs synthesized by Green synthesis using siderophore, were characterized by X-ray diffraction (XRD), UV- spectroscopy (UV), Fourier Transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The characterization techniques determined the size, morphology and distribution of size of NPs. The effect of temperature as well as incubation period on synthesis of NPs was studied. The biologically synthesized nanoparticles were found to have antimicrobial activity against some Gram positive and Gram negative bacteria and fungi as indicated by the zone of inhibition. By studying physicochemical properties of siderophore and using it as a reducing agent in synthesis of AgNPs will enable us to study its antimicrobial activity against phytopathogens and hence can be used as a biocontrol agent. It will also have plant growth promoting activity exhibited by siderophore.

Keywords: Pseudomonas fluorescence, silver nanoparticle synthesis, antimicrobial, Aspergillus flavus, Pseudomonas aeruginosa.