Comparative Evaluation of Hepatoprotective Activity of Syzygium cumini Leaf and Moringa oleifera Flower Extracts Against Hepatotoxicity Using HepG2 Cell Line

The present study demonstrated that Syzygium cumini leaf and Moringa oleifera flower extracts possess a favourable safety profile in the HepG2 cell line, showing no significant cytotoxicity at concentrations of 6.25-100 µg/mL over 24 h. Preliminary phytochemical screening revealed the presence of phenolic compounds, alkaloids, glycosides and phytosterols in both extracts, constituents associated with antioxidant and hepatoprotective activity. These results support both plants as promising natural sources of hepatoprotective agents, warranting further in-vivo and mechanistic studies to establish their protective efficacy against chemically induced liver injury. Overall, the study strengthens the scientific basis for the traditional use of Moringa oleifera and Syzygium cumini in liver-related ailments and provides a validated baseline safety concentration range for future hepatoprotective and mechanistic investigations, including toxin-challenge models, dose-response biochemical assays and comparative antioxidant enzyme profiling, which together could support the eventual development of a standardized polyherbal hepatoprotective formulation from these two locally available plants.

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