Emerging Trends in Electrospun Polymeric Scaffolds for Tissue Engineering and Regenerative Medicine

The present study successfully demonstrated the bio-functionalization of electrospun PLA nanofiber scaffolds for skin tissue engineering applications. The optimized electrospun PLA scaffold provided a highly porous, extracellular matrix (ECM)-like structure that supported efficient attachment, proliferation, and maintenance of adult murine keratinocytes. Sterilization, collagen coating, and the optimized double enzyme isolation protocol collectively enhanced cell adhesion and growth, resulting in the formation of a confluent epithelial layer with characteristic cobblestone morphology. These findings confirm the excellent biocompatibility of the scaffold and its suitability as a substrate for skin cell culture. The incorporation of Lanolin further improved the surface characteristics of the PLA scaffold by modifying its surface charge and enhancing its bioactivity, as demonstrated through zeta potential analysis. The improved surface properties are expected to promote better cell–material interactions and contribute to enhanced wound healing performance. Overall, the developed electrospun PLA–Lanolin scaffold exhibited favourable physicochemical and biological properties required for engineered skin substitutes. The results indicate that the optimized PLA-based scaffold has significant potential as a biodegradable and biocompatible wound dressing and skin tissue engineering scaffold. This approach provides a promising platform for future development of functional skin substitutes and advanced regenerative therapies, with potential applications in the treatment of burns, chronic wounds, and other skin defects.

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