Estimation of DNA From Healthy and Alternaria Blight Infected Leaves of Sunflower

             These observations are supported by Ekbote (1982), whose studies suggested that the infection of plants by different parasites may lead to pronounced changes in nucleic acid content and composition during pathogenesis. Specifically, he noted that the DNA content in sunflower plants infected by Alternaria blight initially increased, but began to decline after a certain period (25 to 30 days). Estimating DNA from infected versus healthy sunflower leaves reveals critical changes in plant-pathogen interactions. When leaf spot or blight pathogens infect a tissue, the absolute composition of the extracted DNA shifts dynamically. By comparing DNA from healthy and infected leaves, this research allows us to detect the disease at a molecular level before the visible damage ruins the crop. Understanding these genetic differences helps scientists trace how the pathogen grows and spreads inside the plant. Ultimately, this work gives the agricultural community the tools needed to protect sunflower yields, save farmers money, and secure the supply of sunflower oil and seeds.

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