Fermentation of Sugarcane Leaves and Napier Grass for Sustainable Biofuel Production

Bio-oil production has become the main substitute for fossil-based fuels, but it should be available as a cost-effective method for its production. Extraction of bio-oil from sugarcane leaf and Napier grass is the motive of this project. We are going to achieve this by grinding the leaves and Napier grass freshly into a liquid form with Sugarcane Juicer that is going to be inoculated with Saccharomyces Cerevisiae (Brewer’s Yeast). The yeast culture was maintained in YPD (Yeast Peptone Dextrose) agar medium for 72 Hours. Once this Yeast grows, it is coagulated with the Sugarcane leaf and Napier grass extract that separates Bio-ethanol by distillation. Then this Bio-oil can be used in engines. This Bio oil is made because this is cost efficient. It is less pollutive when compared to other gasoline Products. These leaves are mostly thrown out as trash or else it is burnt and the part of the Napier grass is used as cattle food and may also be grown in dry lands with small amount of water. So this could be a method of converting the trash and the Napier grass into one of the valuable resources of fuel for vehicles.