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Paper ID:IJSREM66498

Green Synthesis and optimization of monodisperse silver nano particles using Ocimum santum aqeous leaf extract

Stream/Area:

-

Subject:

-

Paper ID:

IJSREM66498

Volume:

10 |

8 |

Aug 2026

Pages:

1-5

Publication Frequency:

Monthly (12 issues per year)

DOI
Digital Object Identifier
Not assigned

License:International Journal of Scientific Research in Engineering and Management follows the Creative Commons Attribution 4.0 (CC BY 4.0) License, allowing free use and distribution with proper attribution.

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Authors

AA

A Aparna

Department of Pharmaceutical Analysis and QA

SSJ College of Pharmacy for women's

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B

Bharathi.G

Department of Pharmaceutical Analysis

SSJ College of Pharmacy for women's

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MAb

M Ashok babu

Department of Pharmaceutical Analysis and QA

SSJ College of Pharmacy for women's

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PPB

Poorna Parvathi B

Department of Pharmaceutics

SSJ College of Pharmacy for women's

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Abstract

This research article discusses the green synthesis of silver nanoparticles (AgNPs) with the help of aquagel leaf extract of Ocimum sanctum (Tulsi). The study aims to identify phytoconstituents of the Ocimum sanctum extract and their efficiency in forming noble metal nanoparticles. Qualitative phytochemical analysis has shown the presence of large amounts of phenols, reducing sugars, tannins, terpenoids, alkaloids, and glycosides. Also, it was noted that after 30 minutes of reaction time, the silver nitrate color changed from a colorless solution into a yellowish-brown color during the process of synthesis. Additionally, characterization was carried out by means of UV-Vis spectroscopy showing an SPR peak at the wavelength of 430 nm and HR SEM providing polymorphs of AgNPs with dimensions of 10–50 nm. The study has shown that this method is an environmentally friendly and inexpensive method for AgNPs synthesis.

KEYWORDS

Silver nanoparticles Ocimum sanctum (Tulsi) UV-Vis spectroscopy.

Cite This Article

A Aparna, Bharathi.G, M Ashok babu, Poorna Parvathi B, "Green Synthesis and optimization of monodisperse silver nano particles using Ocimum santum aqeous leaf extract," International Journal of Scientific Research in Engineering and Management, vol. 10, no. 8, pp. 1-5, Aug 2026.

Full Article/Conclusion

The current study proposes a simple, inexpensive, and eco-friendly method of green synthesis of silver nanoparticles using the leaf extract of Ocimum sanctum. The presence of a multitude of chemicals in Tulsi, especially phenolic compounds, contributes to effective reduction of silver ions and stabilization of synthesized nanoparticles. The produced AgNPs were confirmed through visual inspection, distinct UV-Vis SPR peak, and SEM which demonstrated uniformity and polytypism with sizes in the range of 10-50 nm. The results indicate the applicability of the synthesized Ocimum sanctum AgNPs in nanobiotech and pharmaceuticals thus paving the way for environmentally-friendly protocol in industrial-scale synthesis of nanoparticles. However, the studies on extraction, characterization and evaluation of toxicity of active components must be carried out. From the technology point of view, these silver nanoparticles have a significant number of applications in medicine.

References

1) Nikhil, S.G., Chaitanya, D.G, Amol., G.J., & Prashant, A.P. (2021). Study of medicinal uses of Ocimum sanctum (Tulsi). J Pharmacogn Phytochem, 10(2), 1427-31. 2)    Rakesh, K.J., William, N.S., & Joyce Kelly, D.S. (2016). Phytoconstituents, Traditional medicinal uses and bioactivities of Tulsi (Ocimum sanctum Linn.): A review. American Journal of Essential Oils and Natural Products, 5, 18-21 3)Buzea C, Pacheco II, Robbie K. Nanomaterials and nanoparticles: sources and Mulvaney P. Nanoscience vs nanotechnology—defining the field. ACS Nano. 2015. 4) Hasan S. A review on nanoparticles: their synthesis and types. Res J Recent Sci. 2015; 2277: 2502. 5)Bratovcic A. Different applications of nanomaterials and their impact on the environment. Int J Mater Sci Eng. 2019; 5:1–7. 6)Gajanan K, Tijare SN. Applications of nanomaterials. Mater Today Proc. 2018;5(1):1093–6. 7) Khmayies SJ. Characterization of nanomaterials. JOM. 2014;66(1):28–9. 8)    Anandalakshmi K, Venugobal, J. (2017). Green Synthesis and Characterization of Silver Nanoparticles Using Ocimum sanctum Leaf Extract and its Antibacterial Activity. Med Chem (Los Angeles) 7: 218-225 and Health Sciences,4(6):2307-2083. 9)           Arora, S., Jain, J., Rajwade, JM., and Paknikar, KM. (2008). (Cellular responses induced by silver nanoparticles: In vitro studies. ToxicolLett, 179:100. 10) Asharani, P., Wu, Y., Gong, Z. and Valiyaveettil, S. (2008). Toxicity of silver nanoparticles in zebrafish models. Nanotechnology, 19: 255102.

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Publication Date

8/4/2026

Peer Review Type

Double-Blind Peer Review

Review Rounds: 2

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