Invitro Regeneration of Banana by Changing in Hormonal Concentrations I.E. Auxins & Cytokinins

Invitro Regeneration of Banana by Changing in Hormonal Concentrations I.E. Auxins & Cytokinins

Harish Baratea-1, Harish Bangalkara-2

a : plant tissue culture laboratory, Bochra , Akola

ABSTRACT: - The study was conducted at the Bochra plant tissue culture laboratory in the Akot district of Akola, Maharashtra. in 2023–2024. Auxins and cytokines' effects on callus induction, shoot, and root regeneration in bananas grown in vitro. One of the major fruit crops and a source of sustenance is the banana. for a million individuals in poor nations. To initiate and multiply shoots, explants (suckers) of banana cultivars were cultivated on Murashige and Skoog (MS) media supplemented with varying doses of BAP and NAA combinations. As people's awareness of bananas' increasing nutritional and therapeutic worth has grown around the world, bananas have become a priority crop. Bananas are traditionally propagated by the vegetative method. However, there are additional issues with this approach, like pests, to look at how varying BAP and NAA concentrations affect the growth and multiplication of shoots in virus-free plants. After 30 days, adventitious plantlets from the culture meristem grew into a glass container in a green bunch of little plantlets. Four BAP level combinations (1.5, 2.0, 2.5, and 3.0)
and to examine its impact on shoot and root proliferation, three different doses of NAA (1.5, 2.0, and 2.5) were applied to the media. Among the various concentrations, the largest shoot growth multiplied by around 6–8 clumps was seen at 2.5 ml/l BAP + 1.5 ml/l NAA. The concentration of 1.5 ml/l BAP + 2.5 ml/l NAA produced the longest shoots per plantlet (0.43, 2.24, 2.64 cm).

Keywords: In vitro, Micro-propagation, Sucker, Banana, Auxin, Cytokine