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Paper ID:10.55041/IJSREM.ISTCESD015

Isolation and Screening of Phosphate Solubilizing Microorganisms from the Rhizosphere of Orange Plants in Kalmeshwar Tahsil Nagpur District, Maharashtra, India

Stream/Area:

-

Subject:

-

Paper ID:

10.55041/IJSREM.ISTCESD015

Volume:

10 |

9 |

Sep 2026

Pages:

1-6

Publication Frequency:

Monthly (12 issues per year)

DOI
Digital Object Identifier
10.55041/ijsrem.ISTCESD015

License:International Journal of Scientific Research in Engineering and Management follows the Creative Commons Attribution 4.0 (CC BY 4.0) License, allowing free use and distribution with proper attribution.

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Authors

DMR

Dhanshree M. Ridhorkar

Department of Molecular Biology and Genetic Engineering

RTMNU, Nagpur

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YB

Y.S. Banginwar

Department of Molecular Biology and Genetic Engineering

RTMNU, Nagpur

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Abstract

Although a significant amount of soil phosphorus is present in insoluble forms that are poorly accessible to plants, phosphorus is a key macronutrient needed for plant growth and development. Insoluble phosphate is transformed into soluble forms that plants may easily use by phosphate-solubilizing microorganisms (PSMs). The goal of the current study was to separate and filter organisms that solubilize phosphate from the rhizosphere soil of orange (Citrus sinensis) plants in the Nagpur District of Maharashtra, India. Phosphate-solubilizing organisms were recovered from rhizospheric soil samples taken from healthy orange plants using serial dilution and culture-based methods. The creation of transparent halos surrounding colonies on Pikovskaya's agar medium was used to screen the isolates for phosphate-solubilizing activity. Phosphate-solubilization efficiency (PSE) was calculated in order to assess phosphate-solubilization ability Based on their physical and biochemical traits; a few effective isolates were further described. Effective phosphate-solubilizing organisms linked to orange plants' rhizosphere were isolated and identified as a result of the investigation. As plant-growth-promoting rhizobacteria (PGPR), these microbes could enhance phosphorus availability and promote sustainable nutrient management in orange farming.

KEYWORDS

Phosphate-solubilizing microorganisms; Citrus sinensis; orange rhizosphere; rhizospheric soil; phosphate solubilization; phosphate-solubilization index; plant-growth-promoting rhizobacteria; Nagpur District; biofertilizer.

Cite This Article

Dhanshree M. Ridhorkar, Y.S. Banginwar, "Isolation and Screening of Phosphate Solubilizing Microorganisms from the Rhizosphere of Orange Plants in Kalmeshwar Tahsil Nagpur District, Maharashtra, India," International Journal of Scientific Research in Engineering and Management, vol. 10, no. 9, pp. 1-6, Sep 2026. https://doi.org/10.55041/ijsrem.ISTCESD015

Full Article/Conclusion

The present study demonstrated the occurrence of phosphate-solubilizing microorganisms in the rhizosphere soil of orange (Citrus sinensis) plants in Nagpur District, Maharashtra, India. The microorganisms were isolated using culture-based techniques and screened for phosphate-solubilizing activity using Pikovskaya's agar medium. Formation of clear halos around colonies provided preliminary evidence of phosphate-solubilizing ability. The rhizosphere of orange plants therefore represents a potential source of beneficial phosphate-solubilizing microorganisms. These microorganisms may contribute to phosphorus mobilization and could have potential applications as plant-growth-promoting microorganisms and biofertilizers. However, further quantitative phosphate-solubilization studies, molecular identification, greenhouse experiments and field trials are necessary to confirm their effectiveness and agricultural applicability.

References

1. Vessey JK. Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil. 2003;255:571–586. 2. Gyaneshwar P, Naresh KG, Parekh LJ, Poole PS. Role of soil microorganisms in improving P nutrition of plants. Plant and Soil. 2002;245:83–93. 3. Rodríguez H, Fraga R. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances. 1999;17(4–5):319–339. 4. Khan MS, Zaidi A, Wani PA. Role of phosphate-solubilizing microorganisms in sustainable agriculture—A review. Agronomy for Sustainable Development. 2009;29:135–146. 5. Kloepper JW, Schroth MN. Plant growth-promoting rhizobacteria on radishes. In: Proceedings of the 4th International Conference on Plant Pathogenic Bacteria; 1978; Angers, France. p. 879–882. 6. Zaidi A, Khan MS, Ahemad M, Oves M. Plant growth promotion by phosphate solubilizing bacteria. Acta Microbiologica et Immunologica Hungarica. 2009;56(3–4):263–284. 7. Pikovskaya RI. Mobilization of phosphorus in soil in connection with the vital activity of some microbial species. Mikrobiologiya. 1948;17:362–370. 8. Sharma SB, Sayyed RZ, Trivedi MH, Gobi TA. Phosphate solubilizing microbes: sustainable approach for managing phosphorus deficiency in agricultural soils. SpringerPlus. 2013;2:587.  

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Publication Date

9/29/2026

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