Isolation, Morphological Identification and Molecular Characterization of Cantharellus tropicalis and Evaluation of Its Antibacterial Activity

Cantharellus tropicalis presents itself as a promising fungal species from both a taxonomic and a biotechnological perspective. This study establishes a reliable, low-cost workflow, comprising morphological identification, LETS-based genomic DNA extraction, and UV-Vis/gel-based quality assessment, for characterizing C. tropicalis, and provides preliminary evidence that the fungus possesses antibacterial activity against P. aeruginosa. Given the limited data available on tropical Cantharellus species from India, these findings help fill an important gap and open avenues for further research involving molecular barcoding, genome sequencing, secondary-metabolite profiling and bioprospecting.

1.                Aamir S, Sutar S, Singh SK, Baghela A (2015). A rapid and efficient method of fungal genomic DNA extraction, suitable for PCR-based molecular method. J. Plant Pathology & Quarantine, 5(2), 74-81. 2.                Baghel RK, Sharma R, Pandey AK (2009). Activity of acid phosphatase in the ectomycorrhizal fungus Cantharellus tropicalis under controlled conditions. J. Trop. For. Sci., 21(3), 222-226. 3.                Barros L, Ferreira MJ, Queirós B, Ferreira ICFR, Baptista P (2008). Total phenols, ascorbic acid, β-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chemistry, 103(2), 413-419. 4.                Brundrett MC, Tedersoo L (2018). Evolutionary history of mycorrhizal symbioses and global host plant diversity. New Phytologist, 220(4), 1108-1115. 5.                Buyck B, Hofstetter V (2011). The contribution of tef-1 sequences to species delimitation in the Cantharellus cibarius complex in the southeastern USA. Fungal Diversity, 49, 35-46. 6.                Buyck B, Kauff F, Eyssartier G, Couloux A, Hofstetter V (2014). A multigene world phylogeny for Cantharellus. Fungal Diversity, 64, 101-121. 7.                Fredricks DN, Smith C, Meier A (2005). Comparison of six DNA extraction methods for recovery of fungal DNA as assessed by quantitative PCR. Journal of Clinical Microbiology, 43(10), 5122-5128. 8.                Gardes M, Bruns TD (1993). ITS primers with enhanced specificity for basidiomycetes. Molecular Ecology, 2(2), 113-118. 9.                Halling RE, Bates ST (1998). Cantharellus tropicalis, a new species of chanterelle from the tropics. Mycologia, 90(5), 885-888. 10.             Rosenbaum CN, Ron EZ (2004). DNA extraction from fungi: methodological overview and protocol optimization. Biotechniques, 37(3), 450-456.