OPTIMIZATION OF PHYSIOLOGICAL PARAMETERS FOR EXOPOLYSACCHARIDE PRODUCTION USING Pseudomonas aeruginosa MSSRFV42

ABSTRAT: Exopolysaccharides (EPS) are long-chain polysaccharides containing branched, repeating units of sugars or sugar derivatives such as glucose, fructose, mannose and galactose etc, which are secreted into their surrounding environment during the bacterial growth. Due to their unique properties and vast array of application, isolation and study of new EPS producing microbes is highly concerned issue. In the present study potent exopolysaccharide producing AB4 bacteria (Pseudomonas aeruginosa MSSRFV42) which was previously isolated and screened from marine sample was used. To increase the yield of exopolysaccharide significant physiological parameter were determined Submerged fermentation at pH 10 and temperature of 300C gave maximum yield of EPS after 72 hrs incubation using 1% inoculums in media containing 3.5% NaCl. Outcome of this study will definitely contribute in the elucidation components of AB4 EPS in various industries .