Solution Synthesis and Characterization of Size-Controlled Fe3O4@SiO2 Magnetic Nanoparticles for Nucleic Acid Analysis

Solution Synthesis and Characterization of Size-Controlled Fe3O4@SiO2 Magnetic Nanoparticles for Nucleic Acid Analysis

Rakesh Kumar Gupta1, Shikha Vishwakarma1, Prachi Tadge1* and Sudeshna Ray1*
1Advanced Materials Research Centre, Faculty of Science, Rabindranath Tagore University, Bhopal, India

Abstract - Due to the presence of distinctive magnetic response, a chemically changeable surface, and minimal cytotoxicity, Fe3O4@SiO2 magnetic nanoparticles have attracted significant attention in the biomedical field.  In this work, we report the synthesis of Fe3O4 nanoparticles using a solvothermal mediated method using FeCl3.6H2O, sodium acetate and poly ethylene glycol (PEG-6000). The pH of the suspension found to have a pronounced impact on the size and the shape of the synthesized Fe3O4 NPs. Using a modified Stöber technique, Fe3O4@SiO2 magnetic nanoparticles have been prepared, followed by the synthesis of streptavidin (SA) Immobilized Onto SA-Fe3O4@SiO2 NPs. Due to the effective SA immobilization, the synthesized nanoparticles exhibit a great attractive transporters for nucleic corrosive discovery.