STANDARDISATION OF PATHYADI CHURNA- A POLYHERBAL AYURVEDIC MEDICINE

The present study successfully established an HPTLC fingerprint profile for Pathyadi choorna using silica gel 60 F₂₅₄ plates and a mobile phase comprising chloroform:ethyl acetate:formic acid (5:4:0.2). The chromatographic analysis demonstrated well-resolved, reproducible peaks at both 254 nm and 366 nm, confirming the chemical complexity of the formulation and the consistency of the analytical method. The reproducibility of Rf values and relative peak areas across replicate tracks indicates uniformity of the sample and supports the reliability of the developed fingerprint for routine quality assessment. The detection of multiple UV-absorbing and fluorescent phytoconstituents highlights the presence of diverse secondary metabolites, which may collectively contribute to the biological activity of the formulation. Although specific compound identification was not performed due to the absence of reference standards, the generated HPTLC fingerprint serves as a definitive qualitative reference for identity verification, batch-to-batch consistency evaluation, and detection of adulteration. Overall, the developed method provides a simple, rapid, and reproducible analytical tool that can be effectively employed for the standardization and quality control of Pathyadi choorna in both research and industrial settings.

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