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Paper ID:IJSREM10771

Pomegranate Juice-Mediated Green Synthesis of Copper Nanoparticles and Evaluation of Their Antimicrobial Activity

Stream/Area:

-

Subject:

-

Paper ID:

IJSREM10771

Volume:

5 |

10 |

Oct 2021

Pages:

1-7

Publication Frequency:

Monthly (12 issues per year)

DOI
Digital Object Identifier
10.55041/IJSREM10771

License:International Journal of Scientific Research in Engineering and Management follows the Creative Commons Attribution 4.0 (CC BY 4.0) License, allowing free use and distribution with proper attribution.

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Authors

SENV

S. E. Naina Vinodini

Physics

Government City College (A), Nayapul, Hyderabad

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SRB

Suvarna Ravi Bathe

Physics

Government City College (A), Nayapul, Hyderabad

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Abstract

The present study investigates a green route for the synthesis of copper nanoparticles (CuNPs) using pomegranate (Punica granatum L.) juice as a natural reducing, stabilizing and capping medium, and evaluates the antimicrobial activity of the synthesized nanoparticles. Pomegranate juice contains phytochemicals including flavonoids, proteins, tannins, phenols and terpenoids that can participate in the reduction and stabilization of metal nanoparticles. CuNPs were prepared by adding diluted pomegranate juice to a heated copper oxide precursor under stirring. Two juice volumes, 3 mL and 6 mL, were investigated. The products were characterized by UV–Visible spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). UV–Visible spectra showed absorption features at 220 and 232.5 nm. XRD analysis indicated crystalline Cu-containing nanoparticles, with an average crystallite size of 46.30 nm according to the reported Debye–Scherrer calculation. SEM images showed irregular particles and agglomerated/flaky structures in the approximate 50–100 nm range. EDX analysis confirmed copper as the major element in the samples. Antimicrobial activity was assessed by the agar well-diffusion method against Pseudomonas, Proteus, Staphylococcus and Bacillus, producing reported inhibition zones of 23, 17, 19 and 22 mm, respectively. The findings demonstrate the potential of pomegranate juice as a simple and low-cost biological medium for the preparation of copper nanoparticles with antimicrobial activity.

KEYWORDS

copper nanoparticles; green synthesis; Punica granatum; pomegranate juice; antimicrobial activity; nanotechnology

Cite This Article

S. E. Naina Vinodini, Suvarna Ravi Bathe, "Pomegranate Juice-Mediated Green Synthesis of Copper Nanoparticles and Evaluation of Their Antimicrobial Activity," International Journal of Scientific Research in Engineering and Management, vol. 5, no. 10, pp. 1-7, Oct 2021. https://doi.org/10.55041/IJSREM10771

Full Article/Conclusion

In this study , we successfully synthesized copper nanoparticles(CuNPs) using one-step ,eco-friendly and cost effective green synthesis method. The bio-components present in punica granatum (pomegranate ) juice  served as excellent reducing and stabilisng agent during the synthesis process. The UV-Vis and  XRD analysis confirmed the high purity and cubic crystal structure of the CUNPs phase, with a crystalline size of 46.39 nm and it is in agreement with SEM morphology. The green-syntheised CuNps reveal cubic crystal clusters with different lattice constants. The biosynthesized route is more important than the chemically because it offers a new means for researchers to produce cost-effective ,highly active sites with high purity, non-toxic,eco-friendly and have low energy consumption. These efforts will definitely facilitate the translation of synthesis of CuNPs from the laboaratory scale to large –scale industrial production with the goal of benefitting human health and for sustain the protection of environment with chemicals.  The absorption phenomenon in copper nanoparticles, due to surface plasmon resonance is found to be, prominent at about 220 and 232.5 nm .The XRD indicated the presence of CuNPs with significant peaks at [111]. EDX shows the elemental composition is above 80% in the sample. Through antimicrobial activity CuNPs have explored the long term mechanism in antimicrobial efficacy which would open up new avenues in the field of nanomedicine.

References

1. J.Santhosh Kumar, Happy Agarwal, SoumyaMenon, S.Rajesh Kumar and Venkat Kumar “A biological synthesis of Copper nanoparticles and its potential applications Green Synthesis, CharacterizationandApplicationsofNanoparticles” . https://doi.org/10.1016/B978-0-08-102579-6.00009-5© 2019Elsevier Inc 2. P.NagaPadma, SyedThanveerBanu and S.Chaitanya Kumari “Studies on Green Synthesis of Copper Nanoparticles Using Punicagranatum”ARRB,23(1): 1-10,2018; Articleno. ARRB. 38894 3. Farnosh Tavakoli, Masoud  salavati Niasari and Fatemeh Mohandes “Green synthesis of flower like CuI microstructures composed of trigonal nano structures using pomegranate juice “ Materials letter 100 (2013) 133-136. https://doi.org/10.1016/j.matlet.2013.02.114 4. Anand Murthy,Tegane Deaslegn, Mebrattu kassa,Buzuayehu Abebe and Temesgen Assefa “ Synthesis of Green Copper nanoparticles using medicinal plant Hagenia abyssinica (Brace) JF. Gmel. Leaf Extract: Antimicrobial Properties”  published in Hindavi Journal of nanomaterial Volume 2020, Article ID 3924081, 12 pages  https://doi.org/10.1155/2020/3924081. 5. M. T. Delma and M. Jaya Rajan, “Green synthesis of copper and lead nanoparticles using ZingiberOfficinale stem extract,” International Journal of Scientific and Research Publications, vol. 6, no. 11, pp. 134–137, 2016. 6. S. Shende, A. P. Ingle, A. Gade, and M. Rai, “Green synthesis of copper nanoparticles by Citrus medica Linn. (Idilimbu) juice and its antimicrobial activity,” World Journal of Microbiology and Biotechnology, vol. 31, no. 6, pp. 865–873, 2015. 7. R. Khani, B. Roostaei, G. Bagherzade, and M. Moudi, “Green synthesis of copper nanoparticles by fruit extract of Ziziphusspina-christi (L.) Willd.: application for adsorption of triphenylmethane dye and antibacterial assay,” Journal of Molecular Liquids, vol. 255, pp. 541–549, 2018. 8. P.K.Khanna, S.Gaikwad, P.V.Adhyapak, N.Singh and R.Marimuthu“ Synthesis and characterization of Copper Nanoparticles published in Material letters 61(2007 4711-4714. 9. G.Caroling, M.NithyaPriyadarshini, E.Vinodhini, A.MercyRanjitham , P.Shanthi “ Biosynthesis of Copper nanoparticles using aqueous Guava extract – Characterisation of antibacterial effects” International Journal of Pharmacy and Biological Sciences (e-ISSN 2230-7605). 10.                  S. Thakur, S. Sharma, S. Thakur, and R. Rai, “Green synthesis of copper nano-particles using Asparagus adscendensRoxb. root and leaf extract and their antimicrobial activities,” International Journal of Current Microbiology and Applied Sciences, vol. 7, no. 4, pp. 683–694, 2018.

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Publication Date

10/28/2021

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Review Rounds: 2

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