THE EFFECT OF DIFFERENT PHYSICAL FACTORS ON GROWTH AND MORPHOLOGY OF SIX PATHOGENIC FUNGAL SPECIES
PARVATHY PS, T.V Sajeev, Mallikarjuna Swami, Bibishna Venugopal, Minnu Merson
DEPARTMENT OF MICROBIOLOGY
M E S M K MACKAR PILLAY COLLEGE FOR ADVANCED STUDIES, EDATHALA
Under the guidance of
DR. T V SAJEEV (CHIEF SCIENTIST)
DEPARTMENT OF FOREST ENTAMOLOGY FOREST HEALTH DIVISION
KSCSTE - KERALA FOREST RESEARCH INSTITUTE PEECHI – 680 653, THRISSUR, KERALA
ABSTRACT
The project study was to examine, how some physical factors such as different pH, incubation period, media, light source, sugars on growth and developments of six pathogenic fungi, calonectria, clonostachy, diplodia, fusarium, paramyrothecium, pestalopsis in vitro condition. The growth of fungi was obtained by the daily measurement. The uniform pH ranging from pH3 to pH14, incubation period ranging from 2 to 14 days. The media such as potato dextrose agar, malt extract agar, corn meal agar, oat meal agar, sabraoud dextrose agar. The light sources are white light, green light, blue light and yellow light. The report has shown that the growth of six fungal isolates affected by pH. The selected six fungus grows at the pH ranging from pH 3 to pH 12, but at pH 14 no growth was observed and the pH ranging from pH 5 to pH 10 exhibit good growth except the fusarium sps which also shows well growth in pH 12. The influence of incubation period on the fungal isolates growing on potato dextrose agar media, as a result all six fungal isolates showed initial growth after 2nd day and between 4 to 8th days all fungal isolates were showed approached growth compared to control. After 12th and 14th day the growth of the fungal isolates reach maximum and come to an end. The effect of different media on the growth of fungal isolates giving the report reveals those considerable changes. All media are good for the fungus but the diplodia shows highest growth in malt extract agar and cornmeal agar, calonectria has highest growth in corn meal agar and oat meal agar, potato dextrose agar, cornmeal agar and oat meal agar is suitable for pestalopsis. Fusarium shows highest growth in all media. Sugars are the important physical factor that effects the growth of the fungi. Diplodia fusarium and pestalopsis well grown on glucose and sucrose containing media, dextrose containing media is suitable for calonectria and clonostachy. The paramyrothecium shows good growth in presence of glucose.
