A study on osmotic fragility of human erythrocytes using Tonicity solutions

A study on osmotic fragility of human erythrocytes using  Tonicity solutions

¹Jogu Sumathi, ² Zeeshan Ahmed Jaleeli , ^3* Kaleem Ahmed Jaleeli,⁴Ashish Zade

1. Research Scholar Biophysics Research Laboratory, Department of Physics, Nizam College, Osmania University, Hyderabad, Telangana,
2. MBBS Osmania medical college, Hyderabad,

3* Corresponding Author Assistant Professor, Biophysics Research                                                                                                        Laboratory, Department of Physics, Nizam College, Osmania University, Hyderabad,

Telangana, India.

4 .M.Sc Physics Biophysics Research laboratory, Department of Physics, Nizam College, Osmania university, Hyderabad, India.

Abstract

Planning the osmotic behavior and hemolysis% of human erythrocytes isn't just important clinically, yet it is furthermore considerable in the knowledge of material transfer across biological membranes. The fragile gram piece of the spot of hemolysis was observed as an element of extra-cellular osmolarity. The body of this field is to assess the (hemolysis% and osmotic fragility) tests of "β –thalassemia most important and anemia" patients erythrocytes associated withhealthy subjects and to assess the chance of using any of them as an important screening instrument for "β – thalassemia foremost and frailty" patients. Hemolysis % and osmotic fragility exams were studied for (40 β – thalassemia foremost and 25 anemia) patients contrasted with 20 healthy subjects. There was an increase in the hemolysis % for "β-thalassemia major and anemia"patients by (170 and 273) % alone contrasted with the typical erythrocytes. The fragility curve by utilizing NaCl on "β-thalassemia and anemia" patients’ erythrocytes demonstrated a move towards lower NaCl concentration showing that the normal osmotic fragility H50 (the NaCl fixation delivering half hemolysis) to be (0.1 and 0.2)% of "β – thalassemia major and anemia" patientsErythrocytes separately contrasted with (0.45 %) healthy subjects. In addition to use different concentrations from saline (NaCl) solution, this study tested theosmotic behavior of human RBCs for all studied groups using H2O2 as another osmolytes. The data are different from that spotted with sodiumchloride. Fragility grams of human RBCs showed that with an increase the concentration of H2O2 the H% was increased. Finally, H50 equal to 0.5%,

0.45% for "β – thalassemia major and anemia" patients and 0.5% of healthy subjects.

Keywords: sodium chloride, H2O2, β-thalassemia major, osmotic fragility and red blood cells