Comparative analysis of antimicrobial activity leaf extracts against prevalent Bacterial flora Associated with doorknobs at MIET, Meerut (U.P)

Comparative analysis of antimicrobial activity leaf extracts against prevalent Bacterial flora Associated with doorknobs at MIET, Meerut (U.P)

Arshi Husnain, Muskan, Sonia Sharma, Asad Amir

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Abstract - Microorganisms can be found everywhere, bacteria and fungi contaminate the human body, our houses, workplaces, and our environment. Fortunately, among many billions of bacteria, only 1500 can be dangerous to our health, causing different diseases such as pneumonia or skin infection. The human hand serves as a medium for the propagation of microorganisms from place to place, and from person to person. Although, it is nearly impossible for the hand to be free of microorganisms, as the presence of pathogenic bacteria may lead to chronic or acute illness. Every day, door handles are often hotspots for bacteria, public handles especially because of the frequent and inevitable use of most door handles, it can often be expected that bacteria are present. Many factors determine the suitability and population of bacteria. The material of the handle itself contributes to the growth of bacteria, with most door handles being constructed with stainless steel which is a more suitable home for bacteria. The material affects the time bacteria can survive on door handles; but more so, the temperature and humidity of the surrounding air, depending on these bacteria can thrive anywhere from a few hours to a few weeks. Human hands usually harbor microorganisms both as part of the body's normal flora as well as transient microbes contacted from the environment.  People come into contact with a variety of fomites on a regular basis, and bacterial and fungal infections are on the rise.Investigators looked at the microbial growth connected to the use of door knobs by students at MIET COLLEGE OF EDUCATION BAGHPAT BYPASS MEERUT. 50 door knobs were randomly chosen for microbiological analysis. The samples were examined by means of conventional microbiological techniques. Haemolytic Streptococci, Bacillus species, coagulase-negative Staphylococci, Escherichia coli, Proteus species, and Staphylococcus aureus were among the bacteria that were identified from the earphones. Aspergillus spp., Mucor spp., and Rhizopus spp. were the fungi that were isolated. Staph. aureus, 12 (24%), are among the bacteria that were isolated from the door knobs. A substantial difference in the mean occurrence values of  the isolates from the two groups was shown by statistical analysis (P 0.05). The study came to the conclusion that microbial growth does increase with frequent and continuous use of door knobs in comparison to nonfrequent use of door by comparing the occurrence values of the isolates from the two groups of door knobs users. The isolates found were screened for their antimicrobial activity and rose leaf propanol extract  has the best antimicrobial effect against the isolated microbes.

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Key Word; Microbial Contamination, Door, Handles, Knobs, s bacteria, medicinal plants, antimicrobial activity.