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Stream/Area:
-
Subject:
-
Paper ID:
IJSREM23871
Volume:
7 |
6 |
Jun 2023
Pages:
Publication Frequency:
Monthly (12 issues per year)
License:International Journal of Scientific Research in Engineering and Management follows the Creative Commons Attribution 4.0 (CC BY 4.0) License, allowing free use and distribution with proper attribution.
In order to prevent the COVID-19 virus and other infections, contacting surfaces that come into touch with people and other dangerous bacteria must be sterilised. An inexpensive sterilisation box prototype is created in this study to clean tiny things. The box uses heat and ultraviolet (UV) radiation. Two studies were conducted to evaluate performance. The first step was to sterilise IgG (a model protein that is identical to the spike glycoprotein of the SARS-COV-2) in an incubator using UV and heat. The protein was shown to be effectively unfolded and aggregated after a 15-minute UV incubation at 70 °C. The protein’s hydrodynamic size grew from the native protein’s 5 nm to 171 nm under optimal conditions. Likewise, the OD280 values also increased from 0.17 to 0.78 indicating the exposure of more aromatic moieties and unfolding of the protein. The intrinsic fluorescence measurement and FTIR analyses, which revealed a 70% rise in the protein’s -sheets and a 22% decrease in its -helixes, further validated the protein’s unfolding and aggregation. The SARS-COV-2 was effectively inactivated as evidenced by the constructed box’s ability to alter the protein’s natural structure. Additionally, the clinically important E. coli bacteria as well as bacteria gathered from everyday items had 100% antibacterial efficiency after 15 minutes of incubation at 70 °C inside the chamber. It is the first comprehensive performance study on the effectiveness of combining heat and UV irradiation to kill bacteria and viruses.
KEYWORDS
Publication Date
6/20/2023
Peer Review Type
Double-Blind Peer Review
Review Rounds: 2
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Conflict of Interest
The authors declare no conflict of interest related to this research work.
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