Design And Fabrication of Ultraviolet Disinfection Box for Electronic Product and Different Food Items
Asst. Prof. Shriram Ughade1, Atul L. Khobaragade2 , Mohit V. Thakur3 , Sachin K. Kalaskar4 , Sohan A. Channe5
1Project Guide , Department of Mechanical Engineering, Vidarbha Institute of Technology, Nagpur
2345Students, Department of Mechanical Engineering, Vidarbha Institute of Technology, Nagpur
Abstract-
In order to prevent the COVID-19 virus and other infections, contacting surfaces that come into touch with people and other dangerous bacteria must be sterilised. An inexpensive sterilisation box prototype is created in this study to clean tiny things. The box uses heat and ultraviolet (UV) radiation. Two studies were conducted to evaluate performance. The first step was to sterilise IgG (a model protein that is identical to the spike glycoprotein of the SARS-COV-2) in an incubator using UV and heat. The protein was shown to be effectively unfolded and aggregated after a 15-minute UV incubation at 70 °C. The protein's hydrodynamic size grew from the native protein's 5 nm to 171 nm under optimal conditions. Likewise, the OD280 values also increased from 0.17 to 0.78 indicating the exposure of more aromatic moieties and unfolding of the protein. The intrinsic fluorescence measurement and FTIR analyses, which revealed a 70% rise in the protein's -sheets and a 22% decrease in its -helixes, further validated the protein's unfolding and aggregation. The SARS-COV-2 was effectively inactivated as evidenced by the constructed box's ability to alter the protein's natural structure. Additionally, the clinically important E. coli bacteria as well as bacteria gathered from everyday items had 100% antibacterial efficiency after 15 minutes of incubation at 70 °C inside the chamber. It is the first comprehensive performance study on the effectiveness of combining heat and UV irradiation to kill bacteria and viruses.
Key words: UV rays, Disinfection Box, Covid-19, Protection etc.
